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Molecular profiling of the microbial community and sulfate-reducing bacteria associated with six pilot scale passive sulfate-reducing bioreactors.

紀錄類型:	書目-語言資料,印刷品 : Monograph/item
書名/作者:	Molecular profiling of the microbial community and sulfate-reducing bacteria associated with six pilot scale passive sulfate-reducing bioreactors.
作者:	Babbitt, Aaron Brandon.
面頁冊數:	89 p.
附註:	Source: Masters Abstracts International, Volume: 55-01.
Contained By:	Masters Abstracts International55-01(E).
標題:	Microbiology.
標題:	Biology.
ISBN:	9781321943504
摘要、提要註:	Acid mine drainage (AMD) is an environmental hazard across the world. Passive bioreactors utilizing sulfate-reducing bacteria to remediate AMD impacted sites are a promising solution due to their low cost and minimal maintenance. This study profiled the microbial community associated with six in situ, pilot scale bioreactors that were constructed with varying ratios of simple and complex organic substrate and exposed to AMD. Samples were analyzed nine and fourteen months post assembly to ascertain long-term performance. The overall microbial and sulfate-reducing communities were analyzed by 16S rRNA gene and dsrA gene sequencing, respectively. Over the fourteen-month experiment, the results indicated that the microbial community shifted from one dominated by heterotrophic and fermentative microorganisms utilizing the available substrates to one commonly found in untreated AMD. Thus suggesting a decrease in bioreactor performance over time. The data also indicated that the overall microbial communities within the test bioreactors possessed similar members, but in different abundance. Thus it is unlikely that substrate composition played a significant role in community diversity. At the end of the study period, sulfide measurements suggested that the bioreactor containing the highest amount of complex substrate (Barrel 6) resulted in the greatest stimulation of sulfate reduction. Analysis of the dsrA genes from the community in Barrel 6 suggested that bacteria related to thermophilic sulfate-reducers were responsible for the increased sulfate reduction in this bioreactor.
電子資源:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=1595377

Molecular profiling of the microbial community and sulfate-reducing bacteria associated with six pilot scale passive sulfate-reducing bioreactors.
Babbitt, Aaron Brandon.

Molecular profiling of the microbial community and sulfate-reducing bacteria associated with six pilot scale passive sulfate-reducing bioreactors. - 89 p.

Source: Masters Abstracts International, Volume: 55-01.

Thesis (M.S.)--Southern Illinois University at Carbondale, 2015.

Acid mine drainage (AMD) is an environmental hazard across the world. Passive bioreactors utilizing sulfate-reducing bacteria to remediate AMD impacted sites are a promising solution due to their low cost and minimal maintenance. This study profiled the microbial community associated with six in situ, pilot scale bioreactors that were constructed with varying ratios of simple and complex organic substrate and exposed to AMD. Samples were analyzed nine and fourteen months post assembly to ascertain long-term performance. The overall microbial and sulfate-reducing communities were analyzed by 16S rRNA gene and dsrA gene sequencing, respectively. Over the fourteen-month experiment, the results indicated that the microbial community shifted from one dominated by heterotrophic and fermentative microorganisms utilizing the available substrates to one commonly found in untreated AMD. Thus suggesting a decrease in bioreactor performance over time. The data also indicated that the overall microbial communities within the test bioreactors possessed similar members, but in different abundance. Thus it is unlikely that substrate composition played a significant role in community diversity. At the end of the study period, sulfide measurements suggested that the bioreactor containing the highest amount of complex substrate (Barrel 6) resulted in the greatest stimulation of sulfate reduction. Analysis of the dsrA genes from the community in Barrel 6 suggested that bacteria related to thermophilic sulfate-reducers were responsible for the increased sulfate reduction in this bioreactor.

ISBN: 9781321943504Subjects--Topical Terms:

182563
Microbiology.

Molecular profiling of the microbial community and sulfate-reducing bacteria associated with six pilot scale passive sulfate-reducing bioreactors.
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