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Thermally enhanced bioremediation of LNAPL.

紀錄類型:	書目-語言資料,印刷品 : Monograph/item
書名/作者:	Thermally enhanced bioremediation of LNAPL.
作者:	Zeman, Natalie Rae.
面頁冊數:	139 p.
附註:	Source: Masters Abstracts International, Volume: 51-06.
Contained By:	Masters Abstracts International51-06(E).
標題:	Engineering, Environmental.
標題:	Chemistry, Biochemistry.
ISBN:	9781303152627
摘要、提要註:	Inadvertent releases of petroleum liquids into the environment have led to widespread soil and groundwater contamination. Petroleum liquids, referred to as Light Non-Aqueous Phase Liquid (LNAPL), pose a threat to the environment and human health. The purpose of the research described herein was to evaluate thermally enhanced bioremediation as a sustainable remediation technology for rapid cleanup of LNAPL zones. Thermally enhanced LNAPL attenuation was investigated via a thermal microcosm study that considered six different temperatures: 4°C, 9°C, 22°C, 30°C, 35°C, and 40°C. Microcosms were run for a period of 188 days using soil, water and LNAPL from a decommissioned refinery in Evansville, WY. The soil microcosms simulated anaerobic subsurface conditions where sulfate reduction and methanogenesis were the pathways for biodegradation. To determine the optimal temperature range for thermal stimulation and provide guidance for design of field-scale application, both contaminant degradation and soil microbiology were monitored.
電子資源:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=1539584

Thermally enhanced bioremediation of LNAPL.
Zeman, Natalie Rae.

Thermally enhanced bioremediation of LNAPL. - 139 p.

Source: Masters Abstracts International, Volume: 51-06.

Thesis (M.S.)--Colorado State University, 2013.

Inadvertent releases of petroleum liquids into the environment have led to widespread soil and groundwater contamination. Petroleum liquids, referred to as Light Non-Aqueous Phase Liquid (LNAPL), pose a threat to the environment and human health. The purpose of the research described herein was to evaluate thermally enhanced bioremediation as a sustainable remediation technology for rapid cleanup of LNAPL zones. Thermally enhanced LNAPL attenuation was investigated via a thermal microcosm study that considered six different temperatures: 4°C, 9°C, 22°C, 30°C, 35°C, and 40°C. Microcosms were run for a period of 188 days using soil, water and LNAPL from a decommissioned refinery in Evansville, WY. The soil microcosms simulated anaerobic subsurface conditions where sulfate reduction and methanogenesis were the pathways for biodegradation. To determine the optimal temperature range for thermal stimulation and provide guidance for design of field-scale application, both contaminant degradation and soil microbiology were monitored.

ISBN: 9781303152627Subjects--Topical Terms:

422942
Engineering, Environmental.

Thermally enhanced bioremediation of LNAPL.
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100 1 $a Zeman, Natalie Rae. $3 565580
245 1 0 $a Thermally enhanced bioremediation of LNAPL.
300 $a 139 p.
500 $a Source: Masters Abstracts International, Volume: 51-06.
500 $a Advisers: Susan K. De Long; Thomas Sale.
502 $a Thesis (M.S.)--Colorado State University, 2013.
520 $a Inadvertent releases of petroleum liquids into the environment have led to widespread soil and groundwater contamination. Petroleum liquids, referred to as Light Non-Aqueous Phase Liquid (LNAPL), pose a threat to the environment and human health. The purpose of the research described herein was to evaluate thermally enhanced bioremediation as a sustainable remediation technology for rapid cleanup of LNAPL zones. Thermally enhanced LNAPL attenuation was investigated via a thermal microcosm study that considered six different temperatures: 4°C, 9°C, 22°C, 30°C, 35°C, and 40°C. Microcosms were run for a period of 188 days using soil, water and LNAPL from a decommissioned refinery in Evansville, WY. The soil microcosms simulated anaerobic subsurface conditions where sulfate reduction and methanogenesis were the pathways for biodegradation. To determine the optimal temperature range for thermal stimulation and provide guidance for design of field-scale application, both contaminant degradation and soil microbiology were monitored.
520 $a CH4 and CO2 generation occurred in microcosms at 22°C, 30°C, 35°C and 40°C but was not observed at 4°C and 9°C. The total volume of biogas generated after 188 days of incubation was 19 times higher in microcosms at 22°C and 30°C compared to the microcosms at 35°C. When compared to microcosms at 40°C, the total biogas generated was 3 times higher at both 22°C and 30°C. The onset of CH4 and CO2 production occurred first within the microcosms held at 30°C beginning after 28 days of incubation, and second within microcosms at 22°C beginning after 58 days of incubation. A delay in CH4 and CO2 production was observed within microcosms held at 35°C and 40°C (beginning after 173 and 138 days after incubation) contributing to lower cumulative biogas generation at these temperatures relative to 22°C and 30°C. Microcosms incubated at 4°C, 22°C, 30°C, 35°C and 40°C showed statistically significant biological removal of gasoline range organics (GRO) at the alpha = .05 level over the course of the 188-day incubation period. Biotic removal of GRO was significantly higher at 22°C and 30°C when compared to 4°C and 9°C. The observed removals at 22°C, 30°C, 35°C and 40°C were not statistically different from each other. The biological removal of DRO compounds was found to be statistically significant at 22°C, 30°C, 35°C and 40°C and was significantly higher at 22°C when compared to 4°C and 9°C. The percent biological removal of DRO compounds at these temperatures was not statistically different from each other and ranged from 18-22%. Statistically significant biological degradation of all the BTEX compounds only occurred in microcosms at 22°C and 30°C. Benzene, toluene, and xylene biodegradation was observed to be statistically significant in microcosms at 35°C.
520 $a Phylogenetic analysis of the microbial communities present at 22°C via a 16S rRNA gene archaeal clone library and pyrosequencing revealed the presence of both hydrogenotrophic and acetoclastic methanogens. Pyrosequencing data from microcosms at 4°C, 22°C and 35°C revealed that temperature impacted both archaeal and bacterial community structure. A potential requirement for changes in microbial community structure at the higher temperatures (35°C and 40°C) could explain the observed delay in biogas production.
520 $a The results of the thermal microcosm study led to the development of a pilot study to investigate the efficacy of Sustainable Thermally Enhanced LNAPL Attenuation STELA at the site in Evansville, WY. The thermal pilot study approach and preliminary data is included in this thesis. The outcome and results of the pilot study will be presented in the masters theses of other students.
590 $a School code: 0053.
650 4 $a Engineering, Environmental. $3 422942
650 4 $a Chemistry, Biochemistry. $3 422931
690 $a 0775
690 $a 0487
710 2 $a Colorado State University. $b Civil and Environmental Engineering. $3 565581
773 0 $t Masters Abstracts International $g 51-06(E).
790 $a 0053
791 $a M.S.
792 $a 2013
793 $a English
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=1539584