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Plasmid expressed protein kinase C b...

Beautreau, Denise N. S.

Plasmid expressed protein kinase C beta II (PKCbetaII) peptide that selectively inhibits oncogenic VAL 12 ras-p21 induced Xenopus laevis oocyte maturation.

紀錄類型:	書目-語言資料,印刷品 : Monograph/item
書名/作者:	Plasmid expressed protein kinase C beta II (PKCbetaII) peptide that selectively inhibits oncogenic VAL 12 ras-p21 induced Xenopus laevis oocyte maturation.
作者:	Beautreau, Denise N. S.
面頁冊數:	190 p.
附註:	Source: Masters Abstracts International, Volume: 47-03, page: 1638.
Contained By:	Masters Abstracts International47-03.
標題:	Biology, Molecular.
標題:	Chemistry, Biochemistry.
標題:	Health Sciences, Oncology.
ISBN:	9780549938620
摘要、提要註:	There are several elements associated with the onset of cancer. Among these is the conversion of a proto-oncogene into the oncogenic product. A single point mutation in the ras-p21 protein allows it to become constitutively active in its GTP bound state, and become an oncogenic product. The oncogenic ras-p21 has been found in 30% of all cancer cases and shows a high incidence specifically in pancreatic carcinomas (>80%).
電子資源:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=1460611

Plasmid expressed protein kinase C beta II (PKCbetaII) peptide that selectively inhibits oncogenic VAL 12 ras-p21 induced Xenopus laevis oocyte maturation.
Beautreau, Denise N. S.

Plasmid expressed protein kinase C beta II (PKCbetaII) peptide that selectively inhibits oncogenic VAL 12 ras-p21 induced Xenopus laevis oocyte maturation. - 190 p.

Source: Masters Abstracts International, Volume: 47-03, page: 1638.

Thesis (M.S.)--Long Island University, The Brooklyn Center, 2009.

There are several elements associated with the onset of cancer. Among these is the conversion of a proto-oncogene into the oncogenic product. A single point mutation in the ras-p21 protein allows it to become constitutively active in its GTP bound state, and become an oncogenic product. The oncogenic ras-p21 has been found in 30% of all cancer cases and shows a high incidence specifically in pancreatic carcinomas (>80%).

ISBN: 9780549938620Subjects--Topical Terms:

422925
Biology, Molecular.

Plasmid expressed protein kinase C beta II (PKCbetaII) peptide that selectively inhibits oncogenic VAL 12 ras-p21 induced Xenopus laevis oocyte maturation.
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020 $a 9780549938620
035 $a (UMI)AAI1460611
035 $a AAI1460611
040 $a UMI $c UMI
100 1 $a Beautreau, Denise N. S. $3 423270
245 1 0 $a Plasmid expressed protein kinase C beta II (PKCbetaII) peptide that selectively inhibits oncogenic VAL 12 ras-p21 induced Xenopus laevis oocyte maturation.
300 $a 190 p.
500 $a Source: Masters Abstracts International, Volume: 47-03, page: 1638.
500 $a Adviser: Denise L. Chung.
502 $a Thesis (M.S.)--Long Island University, The Brooklyn Center, 2009.
520 $a There are several elements associated with the onset of cancer. Among these is the conversion of a proto-oncogene into the oncogenic product. A single point mutation in the ras-p21 protein allows it to become constitutively active in its GTP bound state, and become an oncogenic product. The oncogenic ras-p21 has been found in 30% of all cancer cases and shows a high incidence specifically in pancreatic carcinomas (>80%).
520 $a Protein Kinase C has been shown to be involved in the activation pathway of oncogenic ras-p21. Previous studies conducted by Chie et al. (2003) found the Protein Kinase C Beta-2 (PKCbetaII) peptide corresponding to the V5 catalytic region of the Protein Kinase C Beta-2 gene to inhibit oncogenic ras-p21 induced maturation of Xenopus laevis oocytes. A previous study by Edwards (2006) expressed the PKCbetaII peptide in the phrGFP-II-N (N-) vector to overcome the issue of proteolysis with the peptide experiment of Chie et al. (2003).
520 $a This project involved the construction of a plasmid with the DNA sequence encoding the PKCbetaII 645-650 peptide into the pOPRSVI/MCS vector in an attempt to overcome toxic effects caused by the previous vector. The synthesized plasmid was co-injected with Val12 ras-p21 in Xenopus laevis oocytes. The levels of maturation were monitored for a period of 48 hours. The results showed the concentration of 0.3938 mug/muL PKCbetaII plasmid inhibited the oncogenic ras-p21 pathway without having any inhibitory effect on the insulin induced wild-type p21 pathway. The results also showed the enhancement of the inhibitory effect of the PKCbetaII plasmid in the presence of Isopropyl beta-D-1-thiogalactopyranoside (IPTG).
590 $a School code: 0198.
650 4 $a Biology, Molecular. $3 422925
650 4 $a Chemistry, Biochemistry. $3 422931
650 4 $a Health Sciences, Oncology. $3 422938
690 $a 0307
690 $a 0487
690 $a 0992
710 2 $a Long Island University, The Brooklyn Center. $3 423271
773 0 $t Masters Abstracts International $g 47-03.
790 1 0 $a Chung, Denise L., $e advisor
790 $a 0198
791 $a M.S.
792 $a 2009
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=1460611

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