大葉大學圖書館 |

登入

回首頁

Transcriptional regulation of the Ca...

Roush, Sarah Fiorentino.

Transcriptional regulation of the Caenorhabditis elegans let-7 microRNA.

紀錄類型:	書目-語言資料,印刷品 : Monograph/item
書名/作者:	Transcriptional regulation of the Caenorhabditis elegans let-7 microRNA.
作者:	Roush, Sarah Fiorentino.
面頁冊數:	181 p.
附註:	Source: Dissertation Abstracts International, Volume: 70-06, Section: B, page: 3325.
Contained By:	Dissertation Abstracts International70-06B.
標題:	Biology, Molecular.
標題:	Chemistry, Biochemistry.
ISBN:	9781109199512
摘要、提要註:	The let-7 family of microRNAs (miRNAs) are important regulators of developmental timing and cellular differentiation. As a member of the C. elegans heterochronic pathway, let-7 controls cell fate transitions from larval stage 4 (L4) to adulthood by post-transcriptionally down-regulating lineage-abnormal 41 (lin-41) and hunchback-like 1 (hbl-1). Primary let-7 (pri-let-7) transcripts are up-regulated in the L3, yet little is known about what controls this transcriptional up-regulation. I sought factors that either turn on let-7 transcription or keep it repressed until the correct developmental time.
電子資源:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3361591

Transcriptional regulation of the Caenorhabditis elegans let-7 microRNA.
Roush, Sarah Fiorentino.

Transcriptional regulation of the Caenorhabditis elegans let-7 microRNA. - 181 p.

Source: Dissertation Abstracts International, Volume: 70-06, Section: B, page: 3325.

Thesis (Ph.D.)--Yale University, 2009.

The let-7 family of microRNAs (miRNAs) are important regulators of developmental timing and cellular differentiation. As a member of the C. elegans heterochronic pathway, let-7 controls cell fate transitions from larval stage 4 (L4) to adulthood by post-transcriptionally down-regulating lineage-abnormal 41 (lin-41) and hunchback-like 1 (hbl-1). Primary let-7 (pri-let-7) transcripts are up-regulated in the L3, yet little is known about what controls this transcriptional up-regulation. I sought factors that either turn on let-7 transcription or keep it repressed until the correct developmental time.

ISBN: 9781109199512Subjects--Topical Terms:

422925
Biology, Molecular.

Transcriptional regulation of the Caenorhabditis elegans let-7 microRNA.
LDR:03174nam 2200313 4500 001 344859
005 20100910124338.5
008 110817s2009 ||||||||||||||||| ||eng d
020 $a 9781109199512
035 $a (UMI)AAI3361591
035 $a AAI3361591
040 $a UMI $c UMI
100 1 $a Roush, Sarah Fiorentino. $3 423082
245 1 0 $a Transcriptional regulation of the Caenorhabditis elegans let-7 microRNA.
300 $a 181 p.
500 $a Source: Dissertation Abstracts International, Volume: 70-06, Section: B, page: 3325.
500 $a Adviser: Frank J. Slack.
502 $a Thesis (Ph.D.)--Yale University, 2009.
520 $a The let-7 family of microRNAs (miRNAs) are important regulators of developmental timing and cellular differentiation. As a member of the C. elegans heterochronic pathway, let-7 controls cell fate transitions from larval stage 4 (L4) to adulthood by post-transcriptionally down-regulating lineage-abnormal 41 (lin-41) and hunchback-like 1 (hbl-1). Primary let-7 (pri-let-7) transcripts are up-regulated in the L3, yet little is known about what controls this transcriptional up-regulation. I sought factors that either turn on let-7 transcription or keep it repressed until the correct developmental time.
520 $a Here I report that one of let-7's targets, the transcription factor hbl-1, is responsible for inhibiting the transcription of let-7 in specific tissues until the L3 stage. hbl-1 is a known developmental timing regulator and inhibits adult development in larval stages. Therefore, one of hbl-1's important functions is to maintain larval stage fates by inhibiting let-7 expression. Additionally, my results reveal let-7 as the first known target of hbl-1 in C. elegans and suggest a negative feedback loop mechanism for let-7 and hbl-1 in regulating cell differentiation.
520 $a In this work, I further characterize a temporal regulatory element (TRE) in the let-7 promoter and an unknown transcriptional activator designated the temporal regulatory element binding protein (TREB) that binds this DNA sequence. I show that point mutating specific bases in the inverted repeat region of TRE abrogates TREB binding. I contribute to the knowledge of TREB's physical characteristics, including an estimated molecular weight and an isoelectric point. Lastly, I present the examination of candidate TREB proteins and their genetic interactions with let-7.
520 $a The high conservation of let-7 and other members of the C. elegans heterochronic pathway implies that the information presented here may be useful in understanding the transcriptional regulation of let-7 in higher organisms. As let-7s critical role in development and cellular differentiation has implicated its mis-expression as a factor that contributes to many diseases, understanding what regulates its expression could translate into diagnostic tools and treatments for these disease states.
590 $a School code: 0265.
650 4 $a Biology, Molecular. $3 422925
650 4 $a Chemistry, Biochemistry. $3 422931
690 $a 0307
690 $a 0487
710 2 $a Yale University. $t Reports on the course of instruction in Yale college. $3 374489
773 0 $t Dissertation Abstracts International $g 70-06B.
790 1 0 $a Slack, Frank J., $e advisor
790 $a 0265
791 $a Ph.D.
792 $a 2009
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3361591

筆 0 讀者評論

多媒體

多媒體檔案
http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3361591

評論

新增評論分享你的心得

Export

取書館別