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Integration site-based clonality analyses of potency of stem and progenitor cells and safety in human gene and cell therapy.

紀錄類型:	書目-電子資源 : Monograph/item
書名/作者:	Integration site-based clonality analyses of potency of stem and progenitor cells and safety in human gene and cell therapy.
作者:	Cooper, Aaron.
出版者:	Ann Arbor : : ProQuest Dissertations & Theses, , 2016
面頁冊數:	269 p.
附註:	Source: Dissertation Abstracts International, Volume: 77-07(E), Section: B.
Contained By:	Dissertation Abstracts International77-07B(E).
標題:	Bioinformatics.
標題:	Molecular biology.
標題:	Biomedical engineering.
ISBN:	9781339526317
摘要、提要註:	Since their development over thirty years ago, replication incompetent retroviral vectors have become indispensable tools for stable genetic modification in gene therapy and experimental biology (Mann et al. 1983). One notable feature of these vectors is that they irreversibly integrate their genome into the genomic DNA of the target cell, where it is copied along with the cellular genes and passed on to daughter cells when the cell proliferates. Because integration occurs at a random location, each transduced cell within a population of reasonable complexity acquires a retroviral genome at a unique site. The integration process does exhibit some preferences, but the process is still random and most genomic sites are amenable to integration. By using methods that can distinguish between these distinct integration sites (ISs) based on their unique properties, one can enumerate the ISs present in a cellular population. More interestingly, these unique properties can be observed over time as cellular clones expand, contract, differentiate, migrate, die or express other behaviors, allowing one to make inferences about clonal biological potential and proliferative dynamics.

Integration site-based clonality analyses of potency of stem and progenitor cells and safety in human gene and cell therapy.
Cooper, Aaron.

Integration site-based clonality analyses of potency of stem and progenitor cells and safety in human gene and cell therapy. - Ann Arbor : ProQuest Dissertations & Theses, 2016 - 269 p.

Source: Dissertation Abstracts International, Volume: 77-07(E), Section: B.

Thesis (Ph.D.)--University of California, Los Angeles, 2016.

Since their development over thirty years ago, replication incompetent retroviral vectors have become indispensable tools for stable genetic modification in gene therapy and experimental biology (Mann et al. 1983). One notable feature of these vectors is that they irreversibly integrate their genome into the genomic DNA of the target cell, where it is copied along with the cellular genes and passed on to daughter cells when the cell proliferates. Because integration occurs at a random location, each transduced cell within a population of reasonable complexity acquires a retroviral genome at a unique site. The integration process does exhibit some preferences, but the process is still random and most genomic sites are amenable to integration. By using methods that can distinguish between these distinct integration sites (ISs) based on their unique properties, one can enumerate the ISs present in a cellular population. More interestingly, these unique properties can be observed over time as cellular clones expand, contract, differentiate, migrate, die or express other behaviors, allowing one to make inferences about clonal biological potential and proliferative dynamics.

ISBN: 9781339526317Subjects--Topical Terms:

184439
Bioinformatics.

Integration site-based clonality analyses of potency of stem and progenitor cells and safety in human gene and cell therapy.
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