大葉大學圖書館 |

Low-cost methods for molecular characterization of mutant plants[electronic resource] :tissue desiccation, DNA extraction and mutation discovery: protocols /

紀錄類型:	書目-電子資源 : Monograph/item
杜威分類號:	572.82
書名/作者:	Low-cost methods for molecular characterization of mutant plants : tissue desiccation, DNA extraction and mutation discovery: protocols // by Bradley J. Till ... [et al.].
其他作者:	Till, Bradley J.
出版者:	Cham : : Springer International Publishing :, 2015.
面頁冊數:	x, 35 p. : : ill. (some col.), digital ;; 24 cm.
Contained By:	Springer eBooks
標題:	Plant molecular genetics.
標題:	Plant hybridization - Molecular aspects.
標題:	Life Sciences.
標題:	Plant Breeding/Biotechnology.
標題:	Biological Techniques.
標題:	Nucleic Acid Chemistry.
ISBN:	9783319162591 (electronic bk.)
ISBN:	9783319162584 (paper)
內容註:	Introduction -- Health and Safety Considerations -- Sample Collection and Storage -- Low-Cost DNA Extraction -- PCR Amplification for Low-Cost Mutation Discovery -- Enzymatic Mismatch Cleavage and Agarose Gel Evaluation of Samples -- Alternative Enzymology for Mismatch Cleavage for TILLING and Ecotilling: Extraction of Enzymes form Common Weedy Plants -- Example Data -- Conclusions.
摘要、提要註:	This book offers low-cost and rapid molecular assays for the characterization of mutant plant germplasm. Detailed protocols are provided for the desiccation of plant tissues; the extraction of high-quality DNA for downstream applications; the extraction of single-strand-specific nucleases for single nucleotide polymorphism; and small insertion/deletion discovery using standard agarose gel electrophoresis. The methods described can be applied in any laboratory equipped for basic molecular biology and do away with the need for expensive freezers and toxic organic compounds. With the appropriate validation of sample quality and longevity, they can provide sufficient DNA for a variety of molecular applications, such as marker studies and TILLING, at approximately one tenth of the cost per sample when compared to commercial kits.
電子資源:	http://dx.doi.org/10.1007/978-3-319-16259-1

Low-cost methods for molecular characterization of mutant plants[electronic resource] :tissue desiccation, DNA extraction and mutation discovery: protocols /
Low-cost methods for molecular characterization of mutant plantstissue desiccation, DNA extraction and mutation discovery: protocols /[electronic resource] :by Bradley J. Till ... [et al.]. - Cham :Springer International Publishing :2015. - x, 35 p. :ill. (some col.), digital ;24 cm.

Introduction -- Health and Safety Considerations -- Sample Collection and Storage -- Low-Cost DNA Extraction -- PCR Amplification for Low-Cost Mutation Discovery -- Enzymatic Mismatch Cleavage and Agarose Gel Evaluation of Samples -- Alternative Enzymology for Mismatch Cleavage for TILLING and Ecotilling: Extraction of Enzymes form Common Weedy Plants -- Example Data -- Conclusions.

This book offers low-cost and rapid molecular assays for the characterization of mutant plant germplasm. Detailed protocols are provided for the desiccation of plant tissues; the extraction of high-quality DNA for downstream applications; the extraction of single-strand-specific nucleases for single nucleotide polymorphism; and small insertion/deletion discovery using standard agarose gel electrophoresis. The methods described can be applied in any laboratory equipped for basic molecular biology and do away with the need for expensive freezers and toxic organic compounds. With the appropriate validation of sample quality and longevity, they can provide sufficient DNA for a variety of molecular applications, such as marker studies and TILLING, at approximately one tenth of the cost per sample when compared to commercial kits.

ISBN: 9783319162591 (electronic bk.)

Standard No.: 10.1007/978-3-319-16259-1doiSubjects--Topical Terms:

183947
Plant molecular genetics.

LC Class. No.: QK981.4

Dewey Class. No.: 572.82

Low-cost methods for molecular characterization of mutant plants[electronic resource] :tissue desiccation, DNA extraction and mutation discovery: protocols /
LDR:02324nmm a2200337 a 4500 001 438676
003 DE-He213
005 20151202132452.0
006 m d
007 cr nn 008maaau
008 160315s2015 gw s 0 eng d
020 $a 9783319162591 (electronic bk.)
020 $a 9783319162584 (paper)
024 7 $a 10.1007/978-3-319-16259-1 $2 doi
035 $a 978-3-319-16259-1
040 $a GP $c GP
041 0 $a eng
050 4 $a QK981.4
072 7 $a PSTL $2 bicssc
072 7 $a TCB $2 bicssc
072 7 $a SCI011000 $2 bisacsh
072 7 $a SCI01000 $2 bisacsh
082 0 4 $a 572.82 $2 23
090 $a QK981.4 $b .L912 2015
245 0 0 $a Low-cost methods for molecular characterization of mutant plants $h [electronic resource] : $b tissue desiccation, DNA extraction and mutation discovery: protocols / $c by Bradley J. Till ... [et al.].
260 $a Cham : $b Springer International Publishing : $b Imprint: Springer, $c 2015.
300 $a x, 35 p. : $b ill. (some col.), digital ; $c 24 cm.
505 0 $a Introduction -- Health and Safety Considerations -- Sample Collection and Storage -- Low-Cost DNA Extraction -- PCR Amplification for Low-Cost Mutation Discovery -- Enzymatic Mismatch Cleavage and Agarose Gel Evaluation of Samples -- Alternative Enzymology for Mismatch Cleavage for TILLING and Ecotilling: Extraction of Enzymes form Common Weedy Plants -- Example Data -- Conclusions.
520 $a This book offers low-cost and rapid molecular assays for the characterization of mutant plant germplasm. Detailed protocols are provided for the desiccation of plant tissues; the extraction of high-quality DNA for downstream applications; the extraction of single-strand-specific nucleases for single nucleotide polymorphism; and small insertion/deletion discovery using standard agarose gel electrophoresis. The methods described can be applied in any laboratory equipped for basic molecular biology and do away with the need for expensive freezers and toxic organic compounds. With the appropriate validation of sample quality and longevity, they can provide sufficient DNA for a variety of molecular applications, such as marker studies and TILLING, at approximately one tenth of the cost per sample when compared to commercial kits.
650 0 $a Plant molecular genetics. $3 183947
650 0 $a Plant hybridization $x Molecular aspects. $3 626112
650 1 4 $a Life Sciences. $3 463509
650 2 4 $a Plant Breeding/Biotechnology. $3 463547
650 2 4 $a Biological Techniques. $3 464355
650 2 4 $a Nucleic Acid Chemistry. $3 466899
700 1 $a Till, Bradley J. $3 626111
710 2 $a SpringerLink (Online service) $3 463450
773 0 $t Springer eBooks
856 4 0 $u http://dx.doi.org/10.1007/978-3-319-16259-1
950 $a Biomedical and Life Sciences (Springer-11642)