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Transcriptional regulation of magnesium transporters contributes to cellular homeostasis in bacteria.

紀錄類型:	書目-電子資源 : Monograph/item
書名/作者:	Transcriptional regulation of magnesium transporters contributes to cellular homeostasis in bacteria.
作者:	Kriner, Michelle Ann.
出版者:	Ann Arbor : : ProQuest Dissertations & Theses, , 2016
面頁冊數:	251 p.
附註:	Source: Dissertation Abstracts International, Volume: 78-01(E), Section: B.
Contained By:	Dissertation Abstracts International78-01B(E).
標題:	Molecular biology.
標題:	Microbiology.
標題:	Genetics.
ISBN:	9781369157604
摘要、提要註:	Magnesium (Mg2+) is an essential element in biology due to its abundance and unique chemical properties. However, the dynamics of Mg2+ homeostasis and the role of this process in cellular physiology are not well understood. I have characterized two mechanisms in the bacterium Salmonella enterica serovar Typhimurium that regulate expression of Mg2+ transporters. First, structural elements in the mRNA leader of the Mg2+ channel gene corA couple accessibility of a binding site for the transcription termination factor Rho to translation of a short open reading frame, leading to an inverse relationship between corA transcription and translational efficiency. Similar mechanisms regulate all three Mg2+ transporters in Salmonella, indicating that increased Mg2+ uptake may be beneficial during translational impairment. Second, the small molecule alarmone (p)ppGpp represses transcription of all Mg2+ transporter genes in Salmonella to prevent deleterious Mg2+ accumulation during programmed translational arrest. Because Rho and (p)ppGpp both balance transcription and translation---two highly Mg2+-dependent processes---on a cell-wide basis, I propose that Rho- and (p)ppGpp-mediated regulation of Mg2+ uptake stabilizes gene expression at large and thus broadly contributes to cellular homeostasis.

Transcriptional regulation of magnesium transporters contributes to cellular homeostasis in bacteria.
Kriner, Michelle Ann.

Transcriptional regulation of magnesium transporters contributes to cellular homeostasis in bacteria. - Ann Arbor : ProQuest Dissertations & Theses, 2016 - 251 p.

Source: Dissertation Abstracts International, Volume: 78-01(E), Section: B.

Thesis (Ph.D.)--Yale University, 2016.

This item is not available from ProQuest Dissertations & Theses.

Magnesium (Mg2+) is an essential element in biology due to its abundance and unique chemical properties. However, the dynamics of Mg2+ homeostasis and the role of this process in cellular physiology are not well understood. I have characterized two mechanisms in the bacterium Salmonella enterica serovar Typhimurium that regulate expression of Mg2+ transporters. First, structural elements in the mRNA leader of the Mg2+ channel gene corA couple accessibility of a binding site for the transcription termination factor Rho to translation of a short open reading frame, leading to an inverse relationship between corA transcription and translational efficiency. Similar mechanisms regulate all three Mg2+ transporters in Salmonella, indicating that increased Mg2+ uptake may be beneficial during translational impairment. Second, the small molecule alarmone (p)ppGpp represses transcription of all Mg2+ transporter genes in Salmonella to prevent deleterious Mg2+ accumulation during programmed translational arrest. Because Rho and (p)ppGpp both balance transcription and translation---two highly Mg2+-dependent processes---on a cell-wide basis, I propose that Rho- and (p)ppGpp-mediated regulation of Mg2+ uptake stabilizes gene expression at large and thus broadly contributes to cellular homeostasis.

ISBN: 9781369157604Subjects--Topical Terms:

182854
Molecular biology.

Transcriptional regulation of magnesium transporters contributes to cellular homeostasis in bacteria.
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520 $a Magnesium (Mg2+) is an essential element in biology due to its abundance and unique chemical properties. However, the dynamics of Mg2+ homeostasis and the role of this process in cellular physiology are not well understood. I have characterized two mechanisms in the bacterium Salmonella enterica serovar Typhimurium that regulate expression of Mg2+ transporters. First, structural elements in the mRNA leader of the Mg2+ channel gene corA couple accessibility of a binding site for the transcription termination factor Rho to translation of a short open reading frame, leading to an inverse relationship between corA transcription and translational efficiency. Similar mechanisms regulate all three Mg2+ transporters in Salmonella, indicating that increased Mg2+ uptake may be beneficial during translational impairment. Second, the small molecule alarmone (p)ppGpp represses transcription of all Mg2+ transporter genes in Salmonella to prevent deleterious Mg2+ accumulation during programmed translational arrest. Because Rho and (p)ppGpp both balance transcription and translation---two highly Mg2+-dependent processes---on a cell-wide basis, I propose that Rho- and (p)ppGpp-mediated regulation of Mg2+ uptake stabilizes gene expression at large and thus broadly contributes to cellular homeostasis.
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