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Structure determination of HIV-1 Tat/Fluid phase membranes and DMPC pipple phase using X-Ray scattering[electronic resource] /

紀錄類型:	書目-語言資料,印刷品 : Monograph/item
杜威分類號:	574.875
書名/作者:	Structure determination of HIV-1 Tat/Fluid phase membranes and DMPC pipple phase using X-Ray scattering/ by Kiyotaka Akabori.
作者:	Akabori, Kiyotaka.
出版者:	Cham : : Springer International Publishing :, 2015.
面頁冊數:	xx, 168 p. : : ill., digital ;; 24 cm.
Contained By:	Springer eBooks
標題:	Lipid membranes.
標題:	Membranes (Biology) - Fluidity.
標題:	HIV infections.
標題:	X-rays - Scattering.
標題:	Physics.
標題:	Membranes.
標題:	Crystallography.
標題:	Membrane Biology.
標題:	Physical Chemistry.
ISBN:	9783319222103
ISBN:	9783319222097
摘要、提要註:	This Thesis in biological physics has two components, describing the use of X-ray scattering techniques to study the structure of two different stacked lipid membrane systems. The first part focuses on the interaction between a short 11-mer peptide, Tat, which is part of the Tat protein in the HIV-1 virus. Although highly positively charged, the Tat protein has been shown to translocate through hydrocarbon lipid bilayers easily, without requiring the cell's energy, which is counter to its Born self-energy. In this work Tat's location in the headgroup region was demonstrated using a combined X-ray scattering and molecular dynamics approach. Bilayer thinning was observed as well as softening of different membrane mimics due to Tat. It was concluded that Tat's headgroup location, which increases the area/lipid, and its bilayer softening likely reduce the energy barrier for passive translocation. The second part is a rigorous investigation of an enigmatic phase in the phase diagram of the lipid dimyristoylphosphatidylcholine (DMPC) The ripple phase has fascinated many researchers in condensed matter physics and physical chemistry as an example of periodically modulated phases, with many theoretical and simulation papers published. Despite systematic studies over the past three decades, molecular details of the structure were still lacking. By obtaining the highest resolution X-ray data so far, this work revealed the complex nature of the chain packing, as well as confirming that the major side is thicker than the minor side of the saw-tooth ripple structure. The new model shows that the chains in the major arm are tilted with respect to the bilayer normal and that the chains in the minor arm are slightly more disordered than all-trans gel-phase chains, i.e., the chains in the minor arm are more fluid-like. This work provides the highest resolution X-ray structure of the ripple phase to-date.
電子資源:	http://dx.doi.org/10.1007/978-3-319-22210-3

Structure determination of HIV-1 Tat/Fluid phase membranes and DMPC pipple phase using X-Ray scattering[electronic resource] /
Akabori, Kiyotaka.

Structure determination of HIV-1 Tat/Fluid phase membranes and DMPC pipple phase using X-Ray scattering[electronic resource] /by Kiyotaka Akabori. - Cham :Springer International Publishing :2015. - xx, 168 p. :ill., digital ;24 cm. - Springer theses,2190-5053. - Springer theses..

This Thesis in biological physics has two components, describing the use of X-ray scattering techniques to study the structure of two different stacked lipid membrane systems. The first part focuses on the interaction between a short 11-mer peptide, Tat, which is part of the Tat protein in the HIV-1 virus. Although highly positively charged, the Tat protein has been shown to translocate through hydrocarbon lipid bilayers easily, without requiring the cell's energy, which is counter to its Born self-energy. In this work Tat's location in the headgroup region was demonstrated using a combined X-ray scattering and molecular dynamics approach. Bilayer thinning was observed as well as softening of different membrane mimics due to Tat. It was concluded that Tat's headgroup location, which increases the area/lipid, and its bilayer softening likely reduce the energy barrier for passive translocation. The second part is a rigorous investigation of an enigmatic phase in the phase diagram of the lipid dimyristoylphosphatidylcholine (DMPC) The ripple phase has fascinated many researchers in condensed matter physics and physical chemistry as an example of periodically modulated phases, with many theoretical and simulation papers published. Despite systematic studies over the past three decades, molecular details of the structure were still lacking. By obtaining the highest resolution X-ray data so far, this work revealed the complex nature of the chain packing, as well as confirming that the major side is thicker than the minor side of the saw-tooth ripple structure. The new model shows that the chains in the major arm are tilted with respect to the bilayer normal and that the chains in the minor arm are slightly more disordered than all-trans gel-phase chains, i.e., the chains in the minor arm are more fluid-like. This work provides the highest resolution X-ray structure of the ripple phase to-date.

ISBN: 9783319222103

Standard No.: 10.1007/978-3-319-22210-3doiSubjects--Topical Terms:

416408
Lipid membranes.

LC Class. No.: QH602

Dewey Class. No.: 574.875

Structure determination of HIV-1 Tat/Fluid phase membranes and DMPC pipple phase using X-Ray scattering[electronic resource] /
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