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Evolution of viral host recognition through multiple pathways.

紀錄類型:	書目-語言資料,印刷品 : Monograph/item
書名/作者:	Evolution of viral host recognition through multiple pathways.
作者:	Stark, Sara.
面頁冊數:	210 p.
附註:	Source: Dissertation Abstracts International, Volume: 77-04(E), Section: B.
Contained By:	Dissertation Abstracts International77-04B(E).
標題:	Biology.
ISBN:	9781339286976
摘要、提要註:	Most viruses are specialists and have a narrow host range, infecting only a single species or host strain. However, many of the viruses currently responsible for causing emerging infectious diseases are generalists and able to infect a wider host range. Viruses that are able to infect multiple hosts are more likely to continue expanding their host range and infecting additional species. The ability to infect a novel host is a complex process. The first step required to acquire the ability to infect a novel host is host recognition; the host recognition binding protein on the virus must be able to recognize and bind the novel host protein, either in addition to or instead of the original host protein. My study aims to examine this step in more detail using bacteriophages (phages) in order to gain a greater understanding of this process in an attempt to one day predict, prevent, or treat viruses that are likely to expand their host range into humans and cause diseases. I utilize serial passages to perform long term evolution of Lambda, a dsDNA phage often used as a model system. The environment for these passages is made more complex and realistic by the use of multiple host strains and/or the unstructured addition of a second phage, Phi80. These studies examine the dynamics between these two phage populations as they evolve, and demonstrate the presence of multiple evolutionary pathways that are heavily influenced by host environment. Next generation sequencing of some of these evolved phages shows the importance of the often touched upon but rarely examined recombination as a mechanism of changing their host range. Directed recombination reiterates this importance while revealing the ability of recombination to expand the host range of the phage. In conclusion, a more complex environment in the laboratory for evolution studies may reveal the preference of viruses for recombination over point mutations as a method of evolution and changes in host recognition, which is important in the further understanding and prediction of viruses likely to expand their host range and cause emerging infectious diseases.
電子資源:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3738384

Evolution of viral host recognition through multiple pathways.
Stark, Sara.

Evolution of viral host recognition through multiple pathways. - 210 p.

Source: Dissertation Abstracts International, Volume: 77-04(E), Section: B.

Thesis (Ph.D.)--State University of New York at Albany, 2015.

Most viruses are specialists and have a narrow host range, infecting only a single species or host strain. However, many of the viruses currently responsible for causing emerging infectious diseases are generalists and able to infect a wider host range. Viruses that are able to infect multiple hosts are more likely to continue expanding their host range and infecting additional species. The ability to infect a novel host is a complex process. The first step required to acquire the ability to infect a novel host is host recognition; the host recognition binding protein on the virus must be able to recognize and bind the novel host protein, either in addition to or instead of the original host protein. My study aims to examine this step in more detail using bacteriophages (phages) in order to gain a greater understanding of this process in an attempt to one day predict, prevent, or treat viruses that are likely to expand their host range into humans and cause diseases. I utilize serial passages to perform long term evolution of Lambda, a dsDNA phage often used as a model system. The environment for these passages is made more complex and realistic by the use of multiple host strains and/or the unstructured addition of a second phage, Phi80. These studies examine the dynamics between these two phage populations as they evolve, and demonstrate the presence of multiple evolutionary pathways that are heavily influenced by host environment. Next generation sequencing of some of these evolved phages shows the importance of the often touched upon but rarely examined recombination as a mechanism of changing their host range. Directed recombination reiterates this importance while revealing the ability of recombination to expand the host range of the phage. In conclusion, a more complex environment in the laboratory for evolution studies may reveal the preference of viruses for recombination over point mutations as a method of evolution and changes in host recognition, which is important in the further understanding and prediction of viruses likely to expand their host range and cause emerging infectious diseases.

ISBN: 9781339286976Subjects--Topical Terms:

171887
Biology.

Evolution of viral host recognition through multiple pathways.
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