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Role of extracellular DNA in biofilm...

American University.

Role of extracellular DNA in biofilm formation by Staphylococcus aureus.

紀錄類型:	書目-語言資料,印刷品 : Monograph/item
書名/作者:	Role of extracellular DNA in biofilm formation by Staphylococcus aureus.
作者:	Farmer, Jackson.
面頁冊數:	52 p.
附註:	Source: Masters Abstracts International, Volume: 55-01.
Contained By:	Masters Abstracts International55-01(E).
標題:	Biology.
標題:	Microbiology.
ISBN:	9781321990577
摘要、提要註:	Biofilms are communities of bacteria, surrounded by a self-produced extracellular matrix, growing attached to a tissue or surface. The biofilm mode of growth protects bacteria from cell stressors such as antibiotics and desiccation, and is thought to play a role in up to 80% of human infections. The biofilm matrix is composed of polymers such as proteinaceous pili, exopolysaccharides and double-stranded DNA. This study investigated the role of extracellular DNA (eDNA) in biofilms formed by Staphylococcus aureus, an important human pathogen. By using a microtiter plate crystal violet binding assay, agarose gel electrophoresis, and confocal scanning laser microscopy, we found that eDNA plays an important structural and adhesive role in S. aureus biofilms. We further found that DNase is a potent inhibitor of S. aureus biofilm formation and found that many environmental bacteria produce DNases that inhibit S. aureus biofilm formation. The results of these studies confirm that eDNA mediates cell-to-cell interactions in bacterial biofilms and suggest that eDNA warrants further consideration as an antimicrobial drug target.
電子資源:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=1596939

Role of extracellular DNA in biofilm formation by Staphylococcus aureus.
Farmer, Jackson.

Role of extracellular DNA in biofilm formation by Staphylococcus aureus. - 52 p.

Source: Masters Abstracts International, Volume: 55-01.

Thesis (M.S.)--American University, 2015.

Biofilms are communities of bacteria, surrounded by a self-produced extracellular matrix, growing attached to a tissue or surface. The biofilm mode of growth protects bacteria from cell stressors such as antibiotics and desiccation, and is thought to play a role in up to 80% of human infections. The biofilm matrix is composed of polymers such as proteinaceous pili, exopolysaccharides and double-stranded DNA. This study investigated the role of extracellular DNA (eDNA) in biofilms formed by Staphylococcus aureus, an important human pathogen. By using a microtiter plate crystal violet binding assay, agarose gel electrophoresis, and confocal scanning laser microscopy, we found that eDNA plays an important structural and adhesive role in S. aureus biofilms. We further found that DNase is a potent inhibitor of S. aureus biofilm formation and found that many environmental bacteria produce DNases that inhibit S. aureus biofilm formation. The results of these studies confirm that eDNA mediates cell-to-cell interactions in bacterial biofilms and suggest that eDNA warrants further consideration as an antimicrobial drug target.

ISBN: 9781321990577Subjects--Topical Terms:

171887
Biology.

Role of extracellular DNA in biofilm formation by Staphylococcus aureus.
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020 $a 9781321990577
035 $a (MiAaPQ)AAI1596939
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040 $a MiAaPQ $c MiAaPQ
100 1 $a Farmer, Jackson. $3 629892
245 1 0 $a Role of extracellular DNA in biofilm formation by Staphylococcus aureus.
300 $a 52 p.
500 $a Source: Masters Abstracts International, Volume: 55-01.
500 $a Adviser: Jeff Kaplan.
502 $a Thesis (M.S.)--American University, 2015.
520 $a Biofilms are communities of bacteria, surrounded by a self-produced extracellular matrix, growing attached to a tissue or surface. The biofilm mode of growth protects bacteria from cell stressors such as antibiotics and desiccation, and is thought to play a role in up to 80% of human infections. The biofilm matrix is composed of polymers such as proteinaceous pili, exopolysaccharides and double-stranded DNA. This study investigated the role of extracellular DNA (eDNA) in biofilms formed by Staphylococcus aureus, an important human pathogen. By using a microtiter plate crystal violet binding assay, agarose gel electrophoresis, and confocal scanning laser microscopy, we found that eDNA plays an important structural and adhesive role in S. aureus biofilms. We further found that DNase is a potent inhibitor of S. aureus biofilm formation and found that many environmental bacteria produce DNases that inhibit S. aureus biofilm formation. The results of these studies confirm that eDNA mediates cell-to-cell interactions in bacterial biofilms and suggest that eDNA warrants further consideration as an antimicrobial drug target.
590 $a School code: 0008.
650 4 $a Biology. $3 171887
650 4 $a Microbiology. $3 182563
690 $a 0306
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710 2 $a American University. $b Biology. $3 629893
773 0 $t Masters Abstracts International $g 55-01(E).
790 $a 0008
791 $a M.S.
792 $a 2015
793 $a English
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=1596939

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