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A nucleotide sequence level approach...

Gray, David E.

A nucleotide sequence level approach to detection of lateral gene transfer in eukaryotic genomes.

紀錄類型:	書目-語言資料,印刷品 : Monograph/item
書名/作者:	A nucleotide sequence level approach to detection of lateral gene transfer in eukaryotic genomes.
作者:	Gray, David E.
面頁冊數:	101 p.
附註:	Source: Masters Abstracts International, Volume: 54-05.
Contained By:	Masters Abstracts International54-05(E).
標題:	Bioinformatics.
標題:	Biology.
ISBN:	9781321758030
摘要、提要註:	Detection of lateral gene transfer (LGT) in eukaryotes has previously relied almost exclusively on phylogenetic analysis of amino acid sequences. This study developed a method for detection of eukaryotic LGT at the nucleotide sequence level. A pipeline was constructed consisting of Perl scripts that automate BLAST searches, phylogeny construction, and selection of phylogenetic trees indicating LGT. This pipeline was tested on the genomes of a bdelloid rotifer, Adineta vaga, and the pea aphid, Acyrthosiphon pisum, using known LGT events as positive controls, as well as on a soil fungus, Laccaria bicolor, and a the black cottonwood tree, Populus trichocarpa. Analysis of positive controls showed that LGT detection was possible in both A. pisum and A. vaga. Seventeen previously unknown probable LGTs from bacteria to A. vaga were detected, many of which were pseudogenes, and a known eukaryote to bacteria LGT event was seen in L. bicolor and P. trichocarpa..
電子資源:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=1589005

A nucleotide sequence level approach to detection of lateral gene transfer in eukaryotic genomes.
Gray, David E.

A nucleotide sequence level approach to detection of lateral gene transfer in eukaryotic genomes. - 101 p.

Source: Masters Abstracts International, Volume: 54-05.

Thesis (M.S.)--The University of Alabama in Huntsville, 2015.

Detection of lateral gene transfer (LGT) in eukaryotes has previously relied almost exclusively on phylogenetic analysis of amino acid sequences. This study developed a method for detection of eukaryotic LGT at the nucleotide sequence level. A pipeline was constructed consisting of Perl scripts that automate BLAST searches, phylogeny construction, and selection of phylogenetic trees indicating LGT. This pipeline was tested on the genomes of a bdelloid rotifer, Adineta vaga, and the pea aphid, Acyrthosiphon pisum, using known LGT events as positive controls, as well as on a soil fungus, Laccaria bicolor, and a the black cottonwood tree, Populus trichocarpa. Analysis of positive controls showed that LGT detection was possible in both A. pisum and A. vaga. Seventeen previously unknown probable LGTs from bacteria to A. vaga were detected, many of which were pseudogenes, and a known eukaryote to bacteria LGT event was seen in L. bicolor and P. trichocarpa..

ISBN: 9781321758030Subjects--Topical Terms:

184439
Bioinformatics.

A nucleotide sequence level approach to detection of lateral gene transfer in eukaryotic genomes.
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020 $a 9781321758030
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100 1 $a Gray, David E. $3 629830
245 1 2 $a A nucleotide sequence level approach to detection of lateral gene transfer in eukaryotic genomes.
300 $a 101 p.
500 $a Source: Masters Abstracts International, Volume: 54-05.
500 $a Adviser: Leland J. Cseke.
502 $a Thesis (M.S.)--The University of Alabama in Huntsville, 2015.
520 $a Detection of lateral gene transfer (LGT) in eukaryotes has previously relied almost exclusively on phylogenetic analysis of amino acid sequences. This study developed a method for detection of eukaryotic LGT at the nucleotide sequence level. A pipeline was constructed consisting of Perl scripts that automate BLAST searches, phylogeny construction, and selection of phylogenetic trees indicating LGT. This pipeline was tested on the genomes of a bdelloid rotifer, Adineta vaga, and the pea aphid, Acyrthosiphon pisum, using known LGT events as positive controls, as well as on a soil fungus, Laccaria bicolor, and a the black cottonwood tree, Populus trichocarpa. Analysis of positive controls showed that LGT detection was possible in both A. pisum and A. vaga. Seventeen previously unknown probable LGTs from bacteria to A. vaga were detected, many of which were pseudogenes, and a known eukaryote to bacteria LGT event was seen in L. bicolor and P. trichocarpa..
590 $a School code: 0278.
650 4 $a Bioinformatics. $3 184439
650 4 $a Biology. $3 171887
690 $a 0715
690 $a 0306
710 2 $a The University of Alabama in Huntsville. $b Biological Sciences. $3 629831
773 0 $t Masters Abstracts International $g 54-05(E).
790 $a 0278
791 $a M.S.
792 $a 2015
793 $a English
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=1589005

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