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Antibacterial Activity of Epigallocatechin-3-Gallate (EGCG) on Pseudomonas aeruginosa.

紀錄類型:	書目-語言資料,印刷品 : Monograph/item
書名/作者:	Antibacterial Activity of Epigallocatechin-3-Gallate (EGCG) on Pseudomonas aeruginosa.
作者:	Kanagaratnam, Rashmi.
面頁冊數:	51 p.
附註:	Source: Masters Abstracts International, Volume: 54-06.
Contained By:	Masters Abstracts International54-06(E).
標題:	Microbiology.
標題:	Molecular biology.
標題:	Biology.
ISBN:	9781339056654
摘要、提要註:	Pseudomonas aeruginosa is a gram-negative bacterium causing primarily nosocomial and chronic infections in cystic fibrosis patients. Various antibiotics including beta-lactams, aminoglycosides and fluoroquinolones are used to treat these infections. However, P. aeruginosa can acquire resistance to these antibiotics quickly due to acquisition of resistant plasmids, mutations or its inherent resistance. Epigallocatechin-3-gallate (EGCG; green tea extract) has been reported to have antibacterial effects on mainly gram-positive bacteria. In this study, antibacterial activity of EGCG and its effect on antibiotic susceptibility was examined to understand its usefulness for Pseudomonas treatment. Twenty-two clinical isolates of P. aeruginosa, obtained from Downstate Medical Center (Brooklyn, NY), and a reference strain P. aeruginosa PAO1 were used to determine minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of EGCG. Results revealed that MIC and MBC of all clinical isolates and the wild type PAO1 ranged from 128 to 256 mug/mL and from 256 to 512 mug/mL, respectively. Sub-inhibitory concentrations (256 mug/mL) of EGCG significantly enhanced susceptibility to various antibiotics, in particular, to tetracyclines. Time-kill assay of the reference strain PAO1 and one of clinical isolates showed the sub-inhibitory concentration of EGCG in combination with tetracycline (4 mug/mL) killed 99% of both strains within 12 hours. Fractional inhibitory concentration (FIC) index for the EGCG and tetracycline showed less than 0.5 in both strains, suggesting a synergistic effect between EGCG and tetracycline. Gene-knockout analysis showed that the mexCD-OprJ efflux pump was likely, but the mexAB-OprM efflux pump was unlikely, associated with the EGCG effect on antibiotic susceptibility in P. aeruginosa. Overall results suggested that antibacterial activity of EGCG alone or in combination with tetracycline might be effective to treat P. aeruginosa infections.
電子資源:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=1526666

Antibacterial Activity of Epigallocatechin-3-Gallate (EGCG) on Pseudomonas aeruginosa.
Kanagaratnam, Rashmi.

Antibacterial Activity of Epigallocatechin-3-Gallate (EGCG) on Pseudomonas aeruginosa. - 51 p.

Source: Masters Abstracts International, Volume: 54-06.

Thesis (M.S.)--Long Island University, The Brooklyn Center, 2015.

Pseudomonas aeruginosa is a gram-negative bacterium causing primarily nosocomial and chronic infections in cystic fibrosis patients. Various antibiotics including beta-lactams, aminoglycosides and fluoroquinolones are used to treat these infections. However, P. aeruginosa can acquire resistance to these antibiotics quickly due to acquisition of resistant plasmids, mutations or its inherent resistance. Epigallocatechin-3-gallate (EGCG; green tea extract) has been reported to have antibacterial effects on mainly gram-positive bacteria. In this study, antibacterial activity of EGCG and its effect on antibiotic susceptibility was examined to understand its usefulness for Pseudomonas treatment. Twenty-two clinical isolates of P. aeruginosa, obtained from Downstate Medical Center (Brooklyn, NY), and a reference strain P. aeruginosa PAO1 were used to determine minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of EGCG. Results revealed that MIC and MBC of all clinical isolates and the wild type PAO1 ranged from 128 to 256 mug/mL and from 256 to 512 mug/mL, respectively. Sub-inhibitory concentrations (256 mug/mL) of EGCG significantly enhanced susceptibility to various antibiotics, in particular, to tetracyclines. Time-kill assay of the reference strain PAO1 and one of clinical isolates showed the sub-inhibitory concentration of EGCG in combination with tetracycline (4 mug/mL) killed 99% of both strains within 12 hours. Fractional inhibitory concentration (FIC) index for the EGCG and tetracycline showed less than 0.5 in both strains, suggesting a synergistic effect between EGCG and tetracycline. Gene-knockout analysis showed that the mexCD-OprJ efflux pump was likely, but the mexAB-OprM efflux pump was unlikely, associated with the EGCG effect on antibiotic susceptibility in P. aeruginosa. Overall results suggested that antibacterial activity of EGCG alone or in combination with tetracycline might be effective to treat P. aeruginosa infections.

ISBN: 9781339056654Subjects--Topical Terms:

182563
Microbiology.

Antibacterial Activity of Epigallocatechin-3-Gallate (EGCG) on Pseudomonas aeruginosa.
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