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RNA methodologies[electronic resource] :laboratory guide for isolation and characterization /

紀錄類型:	書目-語言資料,印刷品 : Monograph/item
杜威分類號:	572.88
書名/作者:	RNA methodologies : laboratory guide for isolation and characterization // Robert E. Farrell Jr.
作者:	Farrell, Robert E.
出版者:	Amsterdam ; : Elsevier/Academic Press,, 2010.
面頁冊數:	xxiii, 717 p. : : ill.
附註:	Previous ed.: 2005.
標題:	RNA - Laboratory Manuals. - isolation & purification
標題:	Molecular Biology - Laboratory Manuals. - methods
標題:	RNA - Laboratory Manuals. - physiology
標題:	RNA - Laboratory manuals. - Analysis
ISBN:	9780123747273
ISBN:	0123747279
書目註:	Includes bibliographical references and index.
內容註:	CHAPTER 1: RNA and the Cellular Biochemistry Revisited -- CHAPTER 2: RNA Isolation Strategies -- CHAPTER 3: The Truth about Tissues -- CHAPTER 4: Going Green: RNA and the Molecular Biology of Plants (NEW) -- CHAPTER 5: Isolation of Polyadenylated RNA -- CHAPTER 6: Quality Control for RNA Preparations (EXPANDED) -- CHAPTER 7: Resilient Ribonucleases -- CHAPTER 8: Stringency: Conditions that Influence Nucleic Acid Structure (EXPANDED) -- CHAPTER 9: Electrophoresis of RNA (EXPANDED) -- CHAPTER 10: Photodocumentation and Image Analysis (EXPANDED) -- CHAPTER 11: Northern Analysis -- CHAPTER 12: Nucleic Acid Probe Technology -- CHAPTER 13: Practical Nucleic Acid Hybridization -- CHAPTER 14: Principles of Detection (EXPANDED) -- CHAPTER 15: Quantification of Specific mRNAs by Nuclease Protection -- CHAPTER 16: Analysis of Nuclear RNA (EXPANDED) -- CHAPTER 17: cDNA Synthesis (EXPANDED) -- CHAPTER 18: RT-PCR (NEW) -- CHAPTER 19: Quantitative PCR Techniques -- CHAPTER 20: Functional Genomics and Transcript Profiling (NEW) -- CHAPTER 21: High-Throughput Analysis of Gene Expression -- CHAPTER 22: Non-Array Methods for Global Analysis of Gene Expression (NEW) -- CHAPTER 23: RNA Interference: Targeted Gene Silencing (EXPANDED) -- CHAPTER 24: Genomes, Transcriptomes, Proteomes, and Bioinformatics -- CHAPTER 25: An RNA Paradigm.
摘要、提要註:	This is the fourth edition of the successful laboratory guide which has translated the rich story of riboneucleic acid for over fifteen years. RNA Methodologies 4e presents the latest collection of tested laboratory protocols for the isolation and characterization of eukaryotic and prokaryotic RNA with greater emphasis on transcript profiling, including quantification issues and elucidation of alternative transcription start sites. Collectively the chapters work together providing analysis with clear take-home lessons to assist researchers to understand RNA and to optimize time at the bench. The abundant use of flow charts, tables and graphs are especially helpful in the planning and implementation phases of a project and facilitate learning. 30% new material in this edition includes the addition of RNA isolation protocols including RNA isolation from tissue, expansion of PCR optimization analysis and RNA interference sections, the introduction of a new chapter dealing with the molecular biology of plants, and an expanded glossary.
電子資源:	An electronic book accessible through the World Wide Web; click for information

RNA methodologies[electronic resource] :laboratory guide for isolation and characterization /
Farrell, Robert E.

RNA methodologieslaboratory guide for isolation and characterization /[electronic resource] :Robert E. Farrell Jr. - 4th ed. - Amsterdam ;Elsevier/Academic Press,2010. - xxiii, 717 p. :ill.

Previous ed.: 2005.

Includes bibliographical references and index.

CHAPTER 1: RNA and the Cellular Biochemistry Revisited -- CHAPTER 2: RNA Isolation Strategies -- CHAPTER 3: The Truth about Tissues -- CHAPTER 4: Going Green: RNA and the Molecular Biology of Plants (NEW) -- CHAPTER 5: Isolation of Polyadenylated RNA -- CHAPTER 6: Quality Control for RNA Preparations (EXPANDED) -- CHAPTER 7: Resilient Ribonucleases -- CHAPTER 8: Stringency: Conditions that Influence Nucleic Acid Structure (EXPANDED) -- CHAPTER 9: Electrophoresis of RNA (EXPANDED) -- CHAPTER 10: Photodocumentation and Image Analysis (EXPANDED) -- CHAPTER 11: Northern Analysis -- CHAPTER 12: Nucleic Acid Probe Technology -- CHAPTER 13: Practical Nucleic Acid Hybridization -- CHAPTER 14: Principles of Detection (EXPANDED) -- CHAPTER 15: Quantification of Specific mRNAs by Nuclease Protection -- CHAPTER 16: Analysis of Nuclear RNA (EXPANDED) -- CHAPTER 17: cDNA Synthesis (EXPANDED) -- CHAPTER 18: RT-PCR (NEW) -- CHAPTER 19: Quantitative PCR Techniques -- CHAPTER 20: Functional Genomics and Transcript Profiling (NEW) -- CHAPTER 21: High-Throughput Analysis of Gene Expression -- CHAPTER 22: Non-Array Methods for Global Analysis of Gene Expression (NEW) -- CHAPTER 23: RNA Interference: Targeted Gene Silencing (EXPANDED) -- CHAPTER 24: Genomes, Transcriptomes, Proteomes, and Bioinformatics -- CHAPTER 25: An RNA Paradigm.

This is the fourth edition of the successful laboratory guide which has translated the rich story of riboneucleic acid for over fifteen years. RNA Methodologies 4e presents the latest collection of tested laboratory protocols for the isolation and characterization of eukaryotic and prokaryotic RNA with greater emphasis on transcript profiling, including quantification issues and elucidation of alternative transcription start sites. Collectively the chapters work together providing analysis with clear take-home lessons to assist researchers to understand RNA and to optimize time at the bench. The abundant use of flow charts, tables and graphs are especially helpful in the planning and implementation phases of a project and facilitate learning. 30% new material in this edition includes the addition of RNA isolation protocols including RNA isolation from tissue, expansion of PCR optimization analysis and RNA interference sections, the introduction of a new chapter dealing with the molecular biology of plants, and an expanded glossary.

Electronic reproduction.
Amsterdam :
Elsevier Science & Technology,
2010.

Mode of access: World Wide Web.

ISBN: 9780123747273

Source: 166590:166792Elsevier Science & Technologyhttp://www.sciencedirect.comSubjects--Topical Terms:

428214
RNA
--isolation & purification--Laboratory Manuals.Index Terms--Genre/Form:

336502
Electronic books.

LC Class. No.: QP623 / .F37 2010

Dewey Class. No.: 572.88

National Library of Medicine Call No.: 2009 K-772

RNA methodologies[electronic resource] :laboratory guide for isolation and characterization /
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