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Molecular imaging[electronic resource] :FRET microscopy and spectroscopy /

紀錄類型:	書目-語言資料,印刷品 : Monograph/item
杜威分類號:	570/.28
書名/作者:	Molecular imaging : FRET microscopy and spectroscopy // edited by Ammasi Periasamy, Richard N. Day.
其他作者:	Periasamy, Ammasi.
出版者:	Oxford ; : Published for the American Physiological Society by Oxford University Press,, 2005.
面頁冊數:	xv, 312 p. : : ill. (some col.) ;; 26 cm.
標題:	Fluorescence microscopy.
標題:	Fluorescence spectroscopy.
標題:	Microscopy, Fluorescence.
標題:	Microscopy, Fluorescence - methods.
標題:	Spectrometry, Fluorescence.
標題:	Spectrometry, Fluorescence - methods.
標題:	Fluorescence Resonance Energy Transfer.
標題:	Microscopie de fluorescence.
標題:	Spectroscopie de fluorescence.
ISBN:	9780195177206
ISBN:	0195177207
書目註:	Includes bibliographical references and index.
內容註:	Proteins and the Flow of Information in Cellular Function; Basics of Fluorescence and FRET; An Introducrion to Filters and Mirrors for FRET; FRET Imaging in the Wide-field Microscope; Confocal FRET Microscopy - Study of Clustered Distribution of Receptor-ligand Complexes in Endocytic Membranes; Multiphoton FRET Microscopy for Protein Localization in Tissue; Single-Molecule FRET; FRET Measurements using Multispectral Imaging; Real-time Fluorescence Lifetime Imaging and FRET using Fast Gated Image Intensifiers; Streak FLIM: A Novel Technology for Quantitative FRET Imaging; Time-Correlated Single Photon Counting (TCSPC) FLIM-FRET Microscopy for Protein Localization;Bioluminescence RET (BRET): Techniques and Potential; Quantifying Molecular Interactions with Fluorescence Correlation Spectrosocpy; Mapping Molecular Interactions and Transport in Cell Membranes by Image Correlation Spectroscopy; Time-Correlated Single Photon Counting (TCSPC) FLIM-FRET Microscopy for Protein Localization.
摘要、提要註:	The detection and measurement of the dynamic interactions of proteins within the living cell are critical to our understanding of cell physiology and pathophysiology. With FRET microscopy and spectroscopy techniques, basic and clinical scientists can make such measurements at very high spatial and temporal resolution. But sources of background information about these tools are very limited, so this book fills an important gap. It covers both the basic concepts and theory behind the various FRET microscopy and spectroscopy techniques, and the practical aspects of using the techniques and analyzing the results. The critical tricks for obtaining a good FRET image and precisely quantitating the signals from living specimens at the nanomolecular level are explained. Valuable information about the preparation of biological samples used for FRET image analysis is also provided. The methods covered include different types of microscopy systems and detectors (wide-field, confocal, multi-photon) as well as specialized techniques such as photobleaching FRET, FLIM-FRET microscopy, spectral imaging FRET, single molecule FRET, and time and image correlation spectroscopy. Starting from the basics, the chapters guide readers through the choice of probes to be used for FRET experiments and the selection of the most suitable experimental approaches to address specific biological questions. Up-to-date, consistently organized, and well-illustrated, this unique book will be welcomed by all researchers who wish to use FRET microscopy and spectroscopy techniques.
電子資源:	An electronic book accessible through the World Wide Web; click for information

Molecular imaging[electronic resource] :FRET microscopy and spectroscopy /
Molecular imagingFRET microscopy and spectroscopy /[electronic resource] :edited by Ammasi Periasamy, Richard N. Day. - Oxford ;Published for the American Physiological Society by Oxford University Press,2005. - xv, 312 p. :ill. (some col.) ;26 cm. - The American Physiological Society methods in physiology series. - Methods in physiology series..

Includes bibliographical references and index.

Proteins and the Flow of Information in Cellular Function; Basics of Fluorescence and FRET; An Introducrion to Filters and Mirrors for FRET; FRET Imaging in the Wide-field Microscope; Confocal FRET Microscopy - Study of Clustered Distribution of Receptor-ligand Complexes in Endocytic Membranes; Multiphoton FRET Microscopy for Protein Localization in Tissue; Single-Molecule FRET; FRET Measurements using Multispectral Imaging; Real-time Fluorescence Lifetime Imaging and FRET using Fast Gated Image Intensifiers; Streak FLIM: A Novel Technology for Quantitative FRET Imaging; Time-Correlated Single Photon Counting (TCSPC) FLIM-FRET Microscopy for Protein Localization;Bioluminescence RET (BRET): Techniques and Potential; Quantifying Molecular Interactions with Fluorescence Correlation Spectrosocpy; Mapping Molecular Interactions and Transport in Cell Membranes by Image Correlation Spectroscopy; Time-Correlated Single Photon Counting (TCSPC) FLIM-FRET Microscopy for Protein Localization.

The detection and measurement of the dynamic interactions of proteins within the living cell are critical to our understanding of cell physiology and pathophysiology. With FRET microscopy and spectroscopy techniques, basic and clinical scientists can make such measurements at very high spatial and temporal resolution. But sources of background information about these tools are very limited, so this book fills an important gap. It covers both the basic concepts and theory behind the various FRET microscopy and spectroscopy techniques, and the practical aspects of using the techniques and analyzing the results. The critical tricks for obtaining a good FRET image and precisely quantitating the signals from living specimens at the nanomolecular level are explained. Valuable information about the preparation of biological samples used for FRET image analysis is also provided. The methods covered include different types of microscopy systems and detectors (wide-field, confocal, multi-photon) as well as specialized techniques such as photobleaching FRET, FLIM-FRET microscopy, spectral imaging FRET, single molecule FRET, and time and image correlation spectroscopy. Starting from the basics, the chapters guide readers through the choice of probes to be used for FRET experiments and the selection of the most suitable experimental approaches to address specific biological questions. Up-to-date, consistently organized, and well-illustrated, this unique book will be welcomed by all researchers who wish to use FRET microscopy and spectroscopy techniques.

Electronic reproduction.
Amsterdam :
Elsevier Science & Technology,
2007.

Mode of access: World Wide Web.

ISBN: 9780195177206

Source: 137033:137168Elsevier Science & Technologyhttp://www.sciencedirect.comSubjects--Topical Terms:

428096
Fluorescence microscopy.
Index Terms--Genre/Form:

336502
Electronic books.

LC Class. No.: QH212.F55 / M64 2005eb

Dewey Class. No.: 570/.28

National Agricultural Library Call No.: QH212.F55 / M64 2005

Molecular imaging[electronic resource] :FRET microscopy and spectroscopy /
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020 $a 0195177207
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035 $a ocn162131550
037 $a 137033:137168 $b Elsevier Science & Technology $n http://www.sciencedirect.com
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070 1 $a QH212.F55 $b M64 2005
082 0 4 $a 570/.28 $2 22
245 0 0 $a Molecular imaging $h [electronic resource] : $b FRET microscopy and spectroscopy / $c edited by Ammasi Periasamy, Richard N. Day.
260 $a Oxford ; $a New York : $b Published for the American Physiological Society by Oxford University Press, $c 2005.
300 $a xv, 312 p. : $b ill. (some col.) ; $c 26 cm.
490 1 $a The American Physiological Society methods in physiology series
504 $a Includes bibliographical references and index.
505 0 $a Proteins and the Flow of Information in Cellular Function; Basics of Fluorescence and FRET; An Introducrion to Filters and Mirrors for FRET; FRET Imaging in the Wide-field Microscope; Confocal FRET Microscopy - Study of Clustered Distribution of Receptor-ligand Complexes in Endocytic Membranes; Multiphoton FRET Microscopy for Protein Localization in Tissue; Single-Molecule FRET; FRET Measurements using Multispectral Imaging; Real-time Fluorescence Lifetime Imaging and FRET using Fast Gated Image Intensifiers; Streak FLIM: A Novel Technology for Quantitative FRET Imaging; Time-Correlated Single Photon Counting (TCSPC) FLIM-FRET Microscopy for Protein Localization;Bioluminescence RET (BRET): Techniques and Potential; Quantifying Molecular Interactions with Fluorescence Correlation Spectrosocpy; Mapping Molecular Interactions and Transport in Cell Membranes by Image Correlation Spectroscopy; Time-Correlated Single Photon Counting (TCSPC) FLIM-FRET Microscopy for Protein Localization.
520 $a The detection and measurement of the dynamic interactions of proteins within the living cell are critical to our understanding of cell physiology and pathophysiology. With FRET microscopy and spectroscopy techniques, basic and clinical scientists can make such measurements at very high spatial and temporal resolution. But sources of background information about these tools are very limited, so this book fills an important gap. It covers both the basic concepts and theory behind the various FRET microscopy and spectroscopy techniques, and the practical aspects of using the techniques and analyzing the results. The critical tricks for obtaining a good FRET image and precisely quantitating the signals from living specimens at the nanomolecular level are explained. Valuable information about the preparation of biological samples used for FRET image analysis is also provided. The methods covered include different types of microscopy systems and detectors (wide-field, confocal, multi-photon) as well as specialized techniques such as photobleaching FRET, FLIM-FRET microscopy, spectral imaging FRET, single molecule FRET, and time and image correlation spectroscopy. Starting from the basics, the chapters guide readers through the choice of probes to be used for FRET experiments and the selection of the most suitable experimental approaches to address specific biological questions. Up-to-date, consistently organized, and well-illustrated, this unique book will be welcomed by all researchers who wish to use FRET microscopy and spectroscopy techniques.
533 $a Electronic reproduction. $b Amsterdam : $c Elsevier Science & Technology, $d 2007. $n Mode of access: World Wide Web. $n System requirements: Web browser. $n Title from title screen (viewed on July 25, 2007). $n Access may be restricted to users at subscribing institutions.
650 0 $a Fluorescence microscopy. $3 428096
650 0 $a Fluorescence spectroscopy. $3 428097
650 2 $a Microscopy, Fluorescence. $3 428098
650 2 $a Microscopy, Fluorescence $x methods. $3 428099
650 2 $a Spectrometry, Fluorescence. $3 428100
650 2 $a Spectrometry, Fluorescence $x methods. $3 428101
650 2 $a Fluorescence Resonance Energy Transfer. $3 428102
650 6 $a Microscopie de fluorescence. $3 428103
650 6 $a Spectroscopie de fluorescence. $3 428104
655 7 $a Electronic books. $2 local $3 336502
700 1 $a Periasamy, Ammasi. $3 428092
700 1 $a Day, Richard N. $3 428093
710 2 $a American Physiological Society (1887- ) $3 428094
710 2 $a ScienceDirect (Online service) $3 365609
776 1 $c Original $z 0195177207 $z 9780195177206 $w (DLC) 2004061702 $w (OCoLC)57406048
830 0 $a Methods in physiology series. $3 428095
856 4 0 $3 ScienceDirect $u http://www.sciencedirect.com/science/book/9780195177206 $z An electronic book accessible through the World Wide Web; click for information
994 $a C0 $b TEF