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Molecular and biomolecular-based nanomaterials: Tubulin and taxol as molecular constituents.

Record Type:	Language materials, printed : Monograph/item
Title/Author:	Molecular and biomolecular-based nanomaterials: Tubulin and taxol as molecular constituents.
Author:	Castro Carmona, Javier Servando.
Description:	116 p.
Notes:	Source: Dissertation Abstracts International, Volume: 70-04, Section: B, page: 2509.
Contained By:	Dissertation Abstracts International70-04B.
Subject:	Biology, Molecular.
Subject:	Chemistry, Biochemistry.
Subject:	Engineering, Materials Science.
ISBN:	9781109127652
[NT 15000229]:	The new field of protein-based nano-technology takes advantage of the complex interactions between proteins to form unique structures with properties that cannot be achieved with traditional components. Microtubules (MTs), self assembled proteinaceous hollow filaments, offer promise in the development of MT-based nano-systems. The compelling need for the controlled assembly of 3D MT arrays is the fundamental motivation for the first part of this research. We report on the morphology of MTs grown in a crowded environment in the form of high viscosity fluids containing agarose and a novel process that enables the assembly of MTs supported by gel-based 3D scaffolds. Our research on MTs and their interaction with other molecules lead us to discover extraordinary spherulitic structures that changed the course of the project. The novel subject situate us into a complicated dilemma that question the nature of MT asters reported in experiments carried out in cells. The second part of this research is focused in the crystallization of Taxol, a MT stabilizing molecule used as anti-cancer drug. It was confirmed via fluorescent and differential interference contrast microscopy that Taxol crystals can be decorated with fluorescent proteins and fluorochromes without perturbing their morphology. We used theoretical calculations to further investigate Taxol-fluorescent agent interactions. Furthermore, the crystallization of Taxol was studied in pure water, aqueous solutions containing tubulin proteins and tubulin-containing agarose gels. We demonstrated that tubulin is able to heterogeneously nucleate Taxol spherulites. To explain the formation of tubulin-Taxol nuclei a new, secondary Taxol-binding site within the tubulin heterodimer is suggested. Results presented in this work are important for in vivo and in vitro microtubule studies due to the possibility of mistaking these Taxol spherulites for microtubule asters. Thus, we are confirming the need for careful interpretation of fluorescence microscopy observations of MT structures when large concentrations of Taxol are used as stabilizing agent in cells.
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3354939

Molecular and biomolecular-based nanomaterials: Tubulin and taxol as molecular constituents.
Castro Carmona, Javier Servando.

Molecular and biomolecular-based nanomaterials: Tubulin and taxol as molecular constituents. - 116 p.

Source: Dissertation Abstracts International, Volume: 70-04, Section: B, page: 2509.

Thesis (Ph.D.)--The University of Arizona, 2009.

The new field of protein-based nano-technology takes advantage of the complex interactions between proteins to form unique structures with properties that cannot be achieved with traditional components. Microtubules (MTs), self assembled proteinaceous hollow filaments, offer promise in the development of MT-based nano-systems. The compelling need for the controlled assembly of 3D MT arrays is the fundamental motivation for the first part of this research. We report on the morphology of MTs grown in a crowded environment in the form of high viscosity fluids containing agarose and a novel process that enables the assembly of MTs supported by gel-based 3D scaffolds. Our research on MTs and their interaction with other molecules lead us to discover extraordinary spherulitic structures that changed the course of the project. The novel subject situate us into a complicated dilemma that question the nature of MT asters reported in experiments carried out in cells. The second part of this research is focused in the crystallization of Taxol, a MT stabilizing molecule used as anti-cancer drug. It was confirmed via fluorescent and differential interference contrast microscopy that Taxol crystals can be decorated with fluorescent proteins and fluorochromes without perturbing their morphology. We used theoretical calculations to further investigate Taxol-fluorescent agent interactions. Furthermore, the crystallization of Taxol was studied in pure water, aqueous solutions containing tubulin proteins and tubulin-containing agarose gels. We demonstrated that tubulin is able to heterogeneously nucleate Taxol spherulites. To explain the formation of tubulin-Taxol nuclei a new, secondary Taxol-binding site within the tubulin heterodimer is suggested. Results presented in this work are important for in vivo and in vitro microtubule studies due to the possibility of mistaking these Taxol spherulites for microtubule asters. Thus, we are confirming the need for careful interpretation of fluorescence microscopy observations of MT structures when large concentrations of Taxol are used as stabilizing agent in cells.

ISBN: 9781109127652Subjects--Topical Terms:

422925
Biology, Molecular.

Molecular and biomolecular-based nanomaterials: Tubulin and taxol as molecular constituents.
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100 1 $a Castro Carmona, Javier Servando. $3 422998
245 1 0 $a Molecular and biomolecular-based nanomaterials: Tubulin and taxol as molecular constituents.
300 $a 116 p.
500 $a Source: Dissertation Abstracts International, Volume: 70-04, Section: B, page: 2509.
500 $a Adviser: Pierre A. Deymier.
502 $a Thesis (Ph.D.)--The University of Arizona, 2009.
520 $a The new field of protein-based nano-technology takes advantage of the complex interactions between proteins to form unique structures with properties that cannot be achieved with traditional components. Microtubules (MTs), self assembled proteinaceous hollow filaments, offer promise in the development of MT-based nano-systems. The compelling need for the controlled assembly of 3D MT arrays is the fundamental motivation for the first part of this research. We report on the morphology of MTs grown in a crowded environment in the form of high viscosity fluids containing agarose and a novel process that enables the assembly of MTs supported by gel-based 3D scaffolds. Our research on MTs and their interaction with other molecules lead us to discover extraordinary spherulitic structures that changed the course of the project. The novel subject situate us into a complicated dilemma that question the nature of MT asters reported in experiments carried out in cells. The second part of this research is focused in the crystallization of Taxol, a MT stabilizing molecule used as anti-cancer drug. It was confirmed via fluorescent and differential interference contrast microscopy that Taxol crystals can be decorated with fluorescent proteins and fluorochromes without perturbing their morphology. We used theoretical calculations to further investigate Taxol-fluorescent agent interactions. Furthermore, the crystallization of Taxol was studied in pure water, aqueous solutions containing tubulin proteins and tubulin-containing agarose gels. We demonstrated that tubulin is able to heterogeneously nucleate Taxol spherulites. To explain the formation of tubulin-Taxol nuclei a new, secondary Taxol-binding site within the tubulin heterodimer is suggested. Results presented in this work are important for in vivo and in vitro microtubule studies due to the possibility of mistaking these Taxol spherulites for microtubule asters. Thus, we are confirming the need for careful interpretation of fluorescence microscopy observations of MT structures when large concentrations of Taxol are used as stabilizing agent in cells.
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650 4 $a Chemistry, Biochemistry. $3 422931
650 4 $a Engineering, Materials Science. $3 422974
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710 2 $a The University of Arizona. $b Materials Science & Engineering. $3 422999
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790 1 0 $a Deymier, Pierre A., $e advisor
790 1 0 $a Seraphin, Supapan $e committee member
790 1 0 $a Loy, Douglas A. $e committee member
790 1 0 $a Yoon, Jeong-Yeol $e committee member
790 $a 0009
791 $a Ph.D.
792 $a 2009
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3354939